| 1. | Construction of staphylococcus epidermidis icac deletion mutant and the phenotype changes of the mutant
敲除突变株的构建及其表型的改变 |
| 2. | The results of superose 6 gel - filtration chromatography and western blot suggest that the deletion mutant cause a gigantic change in global structure of arog
第三部分工作研究了户四g结构上的“ dz ”对称性在反馈抑制中的作用。 |
| 3. | Using erase - a - base system , a series of exoiii progressive deletion mutants were prepared for sequencing dnd cluster . analysis of the sequence revealed that it contained three orfs
为了对dnd功能区域进行序列测定和分析，通过exo缺失实验，获得了38个单向渐减缺失亚克隆。 |
| 4. | Two magnetosome deletion mutants were constructed by conjugative transposon mutagensis and the application of this genetic system . the two magnetosome deletion mutants were named as nm4 and nm21 respectively
并利用此体系，通过接合转座诱变技术，获得了2个磁小体缺失突变株： nm4 、 nm21 。 |
| 5. | The derivatives of phz2104 resulting from the disruption of the three putative orfs respectively by aada can not confer dnd phenotype on zx1 . using erase - a - base system , a series of exoiii progressive deletion mutants were prepared for sequencing of add gene cluster
将add基因簇亚克隆到测序载体pbluescript sk ( + )上，采用exo缺失突变法得到了66个单向递减缺失亚克隆，然后对这些克隆进行测序。 |
| 6. | Compared to 042bm , the noea deletion mutant 042bma - km showed different degree of increase in nodule number , weight of nodule nodule and plant top dry weight on cultivars of putong zihua , baoding , ningxia , baifa and aohan , but decrease on milu . however , this mutant has no significant change in ability to nodulate cultivars of huanghou and zahua . hence , noea is involved in cultivar - specific nodulation
与苜蓿中华根瘤菌042bm相比，敲除noea的突变株042bma - km在普通紫花、保定、宁夏、百发和傲汉苜蓿品种上的结瘤数、根瘤鲜重和植株地上部分的干重都有不同程度的增加，而在秘鲁苜蓿品种上的结瘤数和植株地上部分的干重明显下降，在皇后和美国杂花苜蓿品种上则没有明显的变化。 |
| 7. | The result of blastx shows that one orf is extracellular serine protease precursor gene ; 3 orfs function as regulatory genes ; 5 orfs are involved in secretion pathway ; 2 orfs are related to production of lps ; and the annotation functions of the other 3 orfs are not clearly related to extracellular protease prouduction . marker exchange method was used to study the relationship between the production of protease and the 3 orfs . a deletion mutant of xcc _ 4463 was constructed successfully
Orf注释表明，共中一个基因为胞外蛋白酶结构基因， 3个基因与合成调控有关， 5个与转运分泌有关， 2个与lps合成有关，其余3个orf的注释功能与胞外蛋白酶的关系未见报道，为研究这3个orf与胞外蛋白酶产生的关系，采用同源双交换orf缺失法进行了进一步验证，成功地构建了xcc _ 4463缺失突变体，所得缺失突变体经检测胞外蛋白酶减少，在寄主上致病性降低。 |
| 8. | The deletion mutants were proved to be genetically stable , and thus might be promising strains in industrial production of avermectins b . although s . avermitilis produces eight related components of avermectins , only two components , bla and bib , are available for the medical , veterinary and agricultural fields
该缺失突变是在染色体上通过同源双交换完成的，不会发生进一步的重组，因此突变株性状稳定，在工业生产上具有应用价值。阿维菌素的天然发酵产物共有八个组分，其中只有b1组分的杀虫活性最高，被作为杀虫剂在农业和畜牧业中使用。 |
